Animal Virology by David Baltimore (Eds.)

By David Baltimore (Eds.)

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18. H . F a n and D . B a l t i m o r e , J . Mol. Biol. 80, 93 (1973). H . F a n and P . B e s m e r , J . V i r o l . 15, 836 (1975). H . F a n and N. M u e l l e r - L a n t z s c h , J . V i r o l . (1976) in press. H . F a n and M. Paskind, J . V i r o l . 14, 421 (1974). H . F a n and S. P e n m a n , J . Mol. Biol. 50, 655 (1970). J . B . F l e i s h m a n , R. H. P a i n and R. R. P o r t e r , A r c h . Bioch, Biophys. Suppl. 1, 174 (1962). A. L . J . Gielkens, M. H. L . Salden and H .

15, 836 (1975). H . F a n and N. M u e l l e r - L a n t z s c h , J . V i r o l . (1976) in press. H . F a n and M. Paskind, J . V i r o l . 14, 421 (1974). H . F a n and S. P e n m a n , J . Mol. Biol. 50, 655 (1970). J . B . F l e i s h m a n , R. H. P a i n and R. R. P o r t e r , A r c h . Bioch, Biophys. Suppl. 1, 174 (1962). A. L . J . Gielkens, M. H. L . Salden and H . Bloemendal, P r o c . Nat. Acad. Sei. 7 1 , 1093 (1974). S. Jons son and G. Kronvall, E u r . J . Immunol. 4, 29 (1974).

The labeled protein was then incubated with the anti-p30 a n t i s e r u m in TEN buffer (0. 02 M T r i s , pH 7 . 5% NP-40 for 18 h r at 4° C, and the immune complexes w e r e p r e c i p i ­ tated by the addition of a ten-fold e x c e s s of sheep a n t i - r a b b i t IgG for 18 h r at 4° C. 5% NP-40 (18) at 10,000 r p m for 30 sec in an Eppendorf m i c r o c e n t r i f u g e , followed by analy­ sis by acrylamide slab gel e l e c t r o p h o r e s i s and a u t o r a d i o graphy. The anti-p30 s e r u m precipitated labeled p30 p r o ­ tein but it did not precipitate significant amounts of the other v i r a l p r o t e i n s .

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