By Phillip Clark
Atlas of medical Avian Hematology is a realistic consultant to the hematological features of birds. Illustrated all through with many colour photos, this wealthy source aids interpretation of hematological info, and promotes the exact category of hematological cells and identity of pathological changes.
Covering over a hundred species of birds, the Atlas illustrates the overall hematological features of birds; the hematological adaptations encountered among the several Orders of birds; and the hematological responses to illness of birds, utilizing scientific situations from many species of birds and a number of scientific disorders.
- Describes how you can gather and deal with blood samples from birds to most sensible shield the standard of the blood.
- Highly illustrated, colour advisor to the hematological features of birds
- Case facts and pictures illustrates the avian hematological responses to disease
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Additional info for Atlas of Clinical Avian Hematology
Collection and handling of blood samples 23 Similarly, Hawkey and Dennett (1989) reported blood films may stain with a “bluish” tinge with Romanowsky stains when heparin is used as an anticoagulant. However, addition of less than 40% of the “correct” amount of blood for the EDTA tube also compromised the quality of the staining of the blood film (Robertson & Maxwell 1993). These authors also found unfixed blood films had superior quality staining than blood films fixed in methanol (for 6 minutes) when stained with May-Grünwald and Giemsa stain (Robertson & Maxwell 1990).
A drop of Figure 20 To make a blood film using the “two cover-slip” method: a second cover-slip is then gently placed on the drop of blood and blood is allowed to spread under its weight. The two cover slips are then gently drawn apart to further spread the blood. Collection and handling of blood samples 19 films are mounted on a slide, either in a permanent fashion using mounting medium or in a temporary fashion using immersion oil (with the side containing the blood film down). All of the methods described may be used to produce blood films of sufficient quality to allow fruitful microscopic examination.
Two slide “wedge” method BLOOD FILMS A commonly used technique for making blood films from a wide range of species is the “wedge” method, which is suitable for many situations. To produce a blood film using this method, place a slide on a flat surface (such as a bench top) then place a drop of blood (about a generous “pin-head” size) towards the end of the slide. Stabilize this slide with a digit (of the operator’s non-preferred hand) then hold a second (“spreader”) slide, between thumb and forefinger of the preferred hand, at about 45 degrees to the (horizontal) first slide.