Bacteriophages: Methods and Protocols, Volume 1: Isolation, by K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton,

By K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench (auth.), Martha R.J. Clokie, Andrew M. Kropinski (eds.)

Ranging from the evolution of pathogenicity to oceanic carbon biking, the numerous and sundry roles that bacteriophages play in microbial ecology and evolution have encouraged elevated curiosity in the medical group. Bacteriophages: tools and Protocols pulls jointly the gigantic physique of data and services from best foreign bacteriophage researchers to supply either classical and cutting-edge molecular innovations. With its well-organized modular layout, Volume 1: Isolation, Characterization, and Interactions examines a large number of themes, together with the isolation of phages, morphological and molecular characterization, and interplay with micro organism. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters include short introductions to the topic, lists of the mandatory fabrics and reagents, without difficulty reproducible laboratory protocols, and a Notes part which information tips about troubleshooting and warding off identified pitfalls.

Thorough and state-of-the-art, Bacteriophages: tools and Protocols is a worthy reference for knowledgeable bacteriophage researchers in addition to an simply obtainable advent for beginners to the subject.

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Extra info for Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

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49, 277–300. 6. C. N. E. F. Hatfull. (1999). Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc. Natl. Acad. Sci. USA 96, 2192–2197. 7. Moreira, D. (2000). Multiple independent horizontal transfers of informational genes from bacteria to plasmids and phages: implications for the origin of bacterial replication machinery. Mol. Microbiol. 35: 1–5. 8. F. (2005). Bacteriophages and bacterial virulence, in Bacteriophages: Biology and Applications, (Kutter E.

D. B. Easterbrook. 1985. Two bacteriophages of Clostridium difficile. Journal of Clinical Microbiology 21:251–254. 21. J. C. Fry. 2003. Elevated abundance of bacteriophage infecting bacteria in soil. Applied & Environmental Microbiology 69:285–289. 22. , E. Manini, and A. Dell’Anno. 2002. Higher abundance of bacteria than viruses in deep Mediterranean sediment. Applied & Environmental Microbiology 68:1468–1472. 23. , A. Dell’Anno, M. Serresi, and S. Vanucci. 2001. Determination of virus adundance in marine sediments.

Microbiol. 55, 2206–2213. Chapter 4 Isolation of Cyanophages from Aquatic Environments Andrew D. Millard Abstract Cyanophages are a group of viruses which specifically infect cyanobacteria. The cyanobacteria are predominantly aquatic phototrophic bacteria and the two dominant genera Synechococcus and Prochlorococcus contribute significantly to primary production in the oceans. Cyanophages that infect marine cyanobacteria were first isolated in the early 1990s and it is now known that by lysing their host cells they play an important role in the microbial loop and alter biogeochemical cycles.

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