Biological Aging: Methods and Protocols by Trygve O. Tollefsbol (auth.), Trygve O. Tollefsbol (eds.)

By Trygve O. Tollefsbol (auth.), Trygve O. Tollefsbol (eds.)

Biological getting older: equipment and Protocols investigates a few of the strategies which are laid low with the age of an organism. a number of new instruments for the research of organic getting older were brought lately, and this quantity offers tools and protocols for those new ideas as well as its insurance of confirmed strategies. The editors have rigorously chosen in basic terms these themes which are thought of mainstays of the sphere or are exhibiting promise in revolutionizing this quite new technological know-how. the 3 major components of concentration during this state of the art compendium of organic getting older examine are: tools which are easy to realizing the basic mechanisms of mobile getting older; ideas used to interfere within the getting older procedure; and methods to interpreting the various molecular procedures of organic getting older. Researchers looking new expertise and methods will locate this quantity of great profit as they movement in the direction of new instructions within the fascinating and increasing box of organic getting older.

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Science 292, 1075–1076. 5. , and Dimri, G. P. (2004) Mechanisms of cellular senescence in human and mouse cells. Biogerontology 5, 1–10. 6. Ben-Porath, I. and Weinberg, R. A. (2004) When cells get stressed: an integrative view of cellular senescence. J. Clin. Invest. 113, 8–13. 7. Dimri, G. , et al. (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92, 9363–9367. 8. , Ramsey, M. , et al. (2004) Ink4a/Arf expression is a biomarker of aging.

Counter staining solution: eosin (Sigma). 4. [3H]Thymidine Labeling and Autoradiography 1. [Methyl-3H]thymidine (70–95 Ci/mmol, 1 mCi/mL), (Amersham Biosciences, Piscataway, NJ, cat. no. TRK758). 2. PBS containing 100 mg/L CaCl2 and 100 mg/L MgCl2 (see Note 3). 3. Methanol. 4. Photographic emulsion [NTB2, cat. no. 165 4433(3H), Kodak, Rochester, NY]: the emulsion is diluted 1:2 or 1:3 with distilled water, aliquoted into plastic vials covered with aluminum foil to protect from light, and stored at 4°C.

Because senescent cells are thought to contribute to a subset of age-related pathologies, its abrogation can potentially help in the treatment of these pathologies. On the other hand, because the senescence response is a tumor suppressor mechanism, its induction by therapeutic agents in tumors can facilitate cancer treatment. Thus, the identification of senescent cells is important in studying the effects of both senescence-inducing and senescence-abrogating agents. Senescent cells can be identified by their failure to undergo DNA synthesis under optimal culture conditions, or by genes that are differentially expressed during senescence.

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