Cell Culture by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Ira

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Ira H. Pastan

The significantly acclaimed laboratory general, tools in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. due to the fact 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this day - really a vital book for researchers in all fields of lifestyles sciences.Key beneficial properties* Covers fundamentals in nice element* Laboratory necessities and Media mobilephone CultureTechniques: Monolayers, progress dimension, and Mass tradition

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M. Pike, Health Lab. Sci. 13, 105 (1976). 7 R. L. Dirnmick, W. Vogl, and M. A. Chatigny, in "Biohazards in Biological Research" (A. Hellman, M. N. Oxman, and R. ), p. 246. , Cold Spring Harbor, New York, 1973. s A. G. Wedum, Public Health Rep. 79, 619 (1964). a G. B. Phillips, J. Chem. Educ. 42, Part One, A43; ibid. Part Two, All7 (1965). 10 H. M. " 1, 169 (1969). 11 M. A. Chatigny and D. I. Clinger, in "An Introduction to Experimental Aerobiology" (L. Dimmick and A. B. ), p. 194. Wiley, New York, 1969.

246. , Cold Spring Harbor, New York, 1973. s A. G. Wedum, Public Health Rep. 79, 619 (1964). a G. B. Phillips, J. Chem. Educ. 42, Part One, A43; ibid. Part Two, All7 (1965). 10 H. M. " 1, 169 (1969). 11 M. A. Chatigny and D. I. Clinger, in "An Introduction to Experimental Aerobiology" (L. Dimmick and A. B. ), p. 194. Wiley, New York, 1969. ~2 M. A. Chatigny, Adv. Appl. Microbiol. 3, 131 (1961). [4] SAFETY CONSIDERATIONS 39 tial for creating aerosols. There are a variety of pipetting aids available that range from simple bulb- and piston-actuated systems to more sophisticated devices which contain their own vacuum pumps.

I f so, the cell is considered diploid (or in special c i r c u m s t a n c e s haploid or tetraploid). I f not, it is considered to be aneuploid or heteroploid. Multiplication potential: Does the cell cease to multiply after a limited n u m b e r of divisions in culture (finite life span), or is it a p e r m a n e n t line capable of continuous long-term multiplication? Anchorage dependence: Does the cell require attachment to a substrate for multiplication, or will it also multiply in suspension culture?

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