By Carl Wu, C. David Allis
DNA within the nucleus of plant and animal cells is kept within the kind of chromatin. Chromatin and the chromatin remodelling enzymes play an immense position in gene transcription. *Genetic assays of chromatin amendment and transforming *Histone editing enzymes *ATP-dependent chromatin home improvement enzymes
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Extra resources for Chromatin and Chromatin Remodeling Enzymes Part C
Morgan, B. A. Mittman, and M. M. Smith, Science 247, S41 (1990). 120 M. Braunstein, R. E. Sobel, C. D. Allis, B. M. Turner, and J. R. Broach, Mol. Cell. Biol. 16, 4349 (1996). 121 D. E. Gottschling, Curr. Biol. 10, R708 (2000). 122 A. Hecht, T. Laroche, S. Strahl-Bolsinger, S. M. Gasser, and G. , Cell 80, 583 (1995). 123 Recent work has also pointed to silencing roles for histone H3 lysines 4 and 79, which are methylated by Set1p and Dot1p, respectively (see earlier). Transcriptional silencing may be studied by standard molecular assays for transcription.
Transcriptional output from the reporter gene is then used as a measure of the human protein function. Proteins such as nuclear hormone receptors (reviewed in McEwan139) and p53 (see later) have been studied productively in this way. In other transcriptional assays, the human protein is expressed as a Gal4p DNA binding domain fusion and targeted to a reporter gene containing Gal4p DNA binding sites. Again, transcriptional output from the reporter gene is used as a measure of the human protein function.
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