Ciba Foundation Symposium 98 - Molecular Biology of Egg

Content:
Chapter 1 advent (pages 1–5): J. R. Tata
Chapter 2 Poly(A) RNA of the Egg Cytoplasm: Structural Resemblance to the Nuclear RNA of Somatic Cells (pages 6–24): Eric H. Davidson, Howard T. Jacobs, Terry L. Thomas, Barbara R. Hough?Evans and Roy J. Britten
Chapter three Induction of Chromosome Replication in the course of Maturation of Amphibian Oocytes (pages 25–43): Ronald A. Laskey, Richard M. Harland and Marcel Mechali
Chapter four Retroviruses and Mouse Embryos: A version process within which to check Gene Expression in improvement and Differentiation (pages 44–62): Rudolf Jaenisch
Chapter five The rules of Yolk Protein Gene Expression in Drosophila melanogaster (pages 63–79): Mary Bownes, Maureen Dempster and Mairearad Blair
Chapter 6 Higher?Order Structural Determinants for Expression of the Ovalbumin Gene family members (pages 80–95): William E. Stumph, Melvyn Baez, George M. Lawson, Ming?Jer Tsai and Bert W. O'Malley
Chapter 7 Hormonal law and Expression of Vitellogenin Multigene kin (pages 96–110): Jamshed R. Tata, Tharappel C. James, Cheryl S. Watson, John L. Williams and Alan P. Wolffe
Chapter eight diet provider Proteins in the course of Embryonic improvement in Birds and Mammals (pages 111–136): P. R. Adiga and C. V. Ramana Murty
Chapter nine Steroid and Peptide regulate Mechanisms in Membrane of Xenopus zaevis Oocytes Resuming Meiotic department (pages 137–158): Etienne?Emile Baulieu and Sabine Schorderet?Slatkine
Chapter 10 Nature and motion of the Mediators Inducing Maturation of the Starfish Oocyte (pages 159–177): Haruo Kanatani
Chapter eleven Somatic keep watch over of Protein Synthesis in Mammalian Oocytes in the course of Maturation (pages 178–196): R. M. Moor and J. C. Osborn
Chapter 12 The Legacy from the Oocyte and its position in Controlling Early improvement of the Mouse Embryo (pages 197–227): Hester P.M. Pratt, Virginia N. Bolton and Katy A. Gudgeon
Chapter thirteen The Oocyte as an Endocytic mobile (pages 228–248): R. A. Wallace, Lee Opresko, H. S. Wiley and Kelly Selman
Chapter 14 The Oocyte as a Secretory telephone (pages 249–267): A. Colman, D. Cutler, P. Krieg and G. Valle
Chapter 15 The Molecular foundation of Sperm?Egg interplay within the Sea Urchin (pages 268–296): Daniel P. Rossignol and William J. Lennarz
Chapter sixteen remaining feedback (pages 297–299):

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Extra info for Ciba Foundation Symposium 98 - Molecular Biology of Egg Maturation

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Examples include the Hind I11 fragment of polyoma DNA which lacks the viral replication origin, all six of the Hind I11 fragments of SV40 whether or not they contain the viral origin, and a wide range of prokaryotic plasmids and viruses (Table 2). In summary, we have been unable to find a DNA molecule which fails to replicate when injected into an unfertilized X . laevis egg. This conclusion was reached independently by McTiernan & Stambrook (1980). Furthermore, in all cases that we have been able to test, replication is regulated so that replicated molecules are distinguished from unreplicated molecules in the presence of cycloheximide.

Cortese et a1 (1980) have shown that a D N A polymerase is active in oocytes and is accompanied by sufficient precursors to synthesize complementary strands for at least 500 pg of single-stranded DNA. This potential for D N A replication can be exploited to derive a cell-free system from X . luevis eggs (MCchali & Harland 1982). In this. system complementary DNA strand synthesis occurs with an unusually high efficiency. 15ng of single-stranded D N A can be converted to a complete doublestranded supercoiled form by the extract equivalent of one egg.

This has been shown for the Mov-13 substrain, as animals homozygous at the Mov-13 locus die between Days 13 and 14 of embryogenesis. This suggests that viral integration occurred in a chromosomal region that is active during, and crucial for, embryonic development. 1983 Molecular biology of egg maturation. Pitman Books, London (Ciba Foundation symposium 98) p 44-63 One of the major unresolved problems in biology is that of gene activation during embryogenesis and cellular differentiation. To study mechanisms of gene regulation in embryonic development, we have analysed the interactions of retroviruses with early mouse embryos.

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