Clinical Biochemistry of Domestic Animals by Jiro J Kaneko

By Jiro J Kaneko

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A blood sample for glucose analysis is taken before injection and at 30-minute intervals from the start of injection for 3 hours. A glucose tolerance curve obtained by this method is illustrated in Fig. 12. Since the alimentary tract has been bypassed, phase I or the absorptive phase has, in effect, been eliminated from the resulting curve. Phase I I and phase I I I are similar to those described for the oral G T T . It should be remembered in the use of this test that, in effect, the curve has been shifted to the left, and glucose returns to normal earlier than in the oral test.

38 J . J . Kaneko ADIPOSE MUSCLE Circulation Increased: Glucose Ketone bodies Cholesterol Urea Lipids, FFA Urea Glucose J Ketone bodies Cholesterol Amino acids G-6-P ^ PEP Pyruvate •Ac CO, Fatty acids Fatty acid Glycogen LIVER Fig. 14. Summary of metabolic alterations in tissues of major importance in the diabetic animal. Increased flow in the metabolic pathways are noted by larger arrows. B. HYPERINSULINISM Following the discovery of insulin, a clinical state showing marked similarities to insulin overdosage was recognized as a disease entity in man and termed hyper­ insulinism.

A n immune response is suggested because lymphocytic infiltration is frequently associated with immune processes and lymphocytic infiltration is found in spontaneous diabetes of cattle ( K a n e k o and Rhode, 1964) and man. It is also observed in cattle ( L e Compte et al, 1966) and/or rabbits (Grodsky et al, 1966) immunized with bovine insulin. The high estimates of the incidence of the disease provide an indication of its importance as a clinical consideration. Furthermore, the similarities of the clinical picture of diabetes with other 'wasting diseases showing polyuria and polydypsia attest to the importance of laboratory examinations in the early and accurate diagnosis of diabetes.

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