By Paul D. Boyer
Content material: v. 17. normal gains -- particular enzymes (I). -- v. 18. particular enzymes (II) -- organic procedures
Read or Download Control by Phosphorylation, Part B (Specific Enzymes), 3rd Edition PDF
Similar biology books
For over fifty years the tools in Enzymology sequence has been the severely aclaimed laboratory average and some of the most revered courses within the box of biochemistry. The hugely suitable fabric makes it a vital ebook for researchers in all fields of existence and comparable sciences.
There are numerous aggressive works out there bearing on evolutionary biology, yet this quantity is sort of detailed in its idiographic element targeting Ostracoda seen from a variety of disciplines, a long time and environments. The publication bargains with numerous traces of idiographic biology and palaeontology of Ostracoda and nomothetic trials focusing strongly on evolutionary biology.
With approximately 100 years of in depth research, lipids have confirmed to be an important and ever-more-promising quarter of cellphone organic learn. In Liposomes: equipment and Protocols, major specialists within the comparable fields discover state of the art experimental tools related to all facets of lipids as crucial elements of the cellphone membrane.
Fungi: Biology and purposes, moment Edition€provides a entire remedy of fungi, masking biochemistry, genetics and the scientific and fiscal value of those organisms at introductory point. € without previous wisdom of the topic assumed, the hole chapters supply a large evaluation of the fundamentals of fungal biology, particularly the body structure and genetics of fungi and in addition a brand new bankruptcy at the program of genomics to fungi.
- Gene Transfer and Expression Protocols
- Localization and Orientation in Biology and Engineering
- Evolutionary Biology
- Advances in Bioprocess Technology
- Colloque sur la limite Jurassique-Crétacé. Lyon, Neuchâtel, septembre 1973
- Biology of Invertebrate and Lower Vertebrate Collagens
Extra info for Control by Phosphorylation, Part B (Specific Enzymes), 3rd Edition
B. YEAST There is a growing amount of evidence that implicates phosphorylation-dephosphorylation in the regulation of fructose- 1,6-bisphosphatase from Saccharomyces cerevisiae. This enzyme has been purified to homogeneity from Baker's yeast and consists of a dimer with a subunit molecular weight of 57,000 (190). The specific activity of the yeast enzyme is 46 units/mg which is similar to that of the rat liver enzyme, and it is inhibited by AMP, Fru-2,6-P,, and its substrate Fru-1,6-P,. The inhibition by AMP is noncompetitive and does not exhibit cooperative behavior (176).
6-Phosphofructo-1-Kinase: Possible Role of Phosphorylation in the Control of Enzyme Activity A. LIVER 6-Phosphofructo- 1-kinase has been purified from livers of a number of species (86-98). The rat liver enzyme consists of four apparently identical subunits with a molecular weight of 82,000 (90-92). Like that of heart (99) and muscle (ZOO), it tends to form aggregates with molecular weights of the order of several million (90-92, 101). This aggregation is an equilibrium process influenced by enzyme concentration, the presence of allosteric effectors, the oxidation-reduction state of sulfhydryl groups, and temperature (8, 9, 91, 92, 102).
Inorganic phosphate and a-glycerol-P are competitive inhibitors at low Fru-2,6-P2 concentrations (26), but both effectors are activators at higher Fru-2,6-P2 concentrations where substrate inhibition is seen (5, 6, 14, 25, 26, 49). 4. It has been reported that GTP, and to a lesser extent ATP, activate partially purified fructose-2,6-bisphosphatase(47, 49), but this observation is not confirmed with a homogeneous preparation of the enzyme (M. R. ElMaghrabi and S. J. Pilkis, unpublished results).