Diagnostic Bacteriology Protocols 2nd Edition (Methods in by Louise O'Connor

By Louise O'Connor

Show description

Read or Download Diagnostic Bacteriology Protocols 2nd Edition (Methods in Molecular Biology Vol 345) PDF

Best biology books

Translation Initiation: Extract Systems and Molecular Genetics

For over fifty years the tools in Enzymology sequence has been the significantly aclaimed laboratory usual and essentially the most revered courses within the box of biochemistry. The hugely proper fabric makes it an important book for researchers in all fields of lifestyles and similar sciences.

Evolutionary biology of ostracoda: its fundamentals and applications: Proceedings of the Ninth International Symposium on Ostracoda

There are numerous aggressive works out there pertaining to evolutionary biology, yet this quantity is sort of designated in its idiographic element concentrating on Ostracoda considered from quite a lot of disciplines, a while and environments. The e-book offers with a variety of strains of idiographic biology and palaeontology of Ostracoda and nomothetic trials focusing strongly on evolutionary biology.

Liposomes: Methods and Protocols, Volume 2: Biological Membrane Models

With approximately 100 years of in depth research, lipids have confirmed to be an important and ever-more-promising zone of mobile organic study. In Liposomes: equipment and Protocols, best specialists within the comparable fields discover state of the art experimental tools concerning all features of lipids as crucial parts of the cellphone membrane.

Fungi : biology and applications

Fungi: Biology and functions, moment Edition€provides a accomplished therapy of fungi, masking biochemistry, genetics and the scientific and monetary importance of those organisms at introductory point. € without previous wisdom of the topic assumed, the outlet chapters supply a large review of the fundamentals of fungal biology, particularly the body structure and genetics of fungi and in addition a brand new bankruptcy at the software of genomics to fungi.

Extra info for Diagnostic Bacteriology Protocols 2nd Edition (Methods in Molecular Biology Vol 345)

Sample text

Thus, on-chip PCR allows the single-step amplification and characterization of a DNA sample as a result of separation in liquid- and solid-phase reactions. In contrast to conventional PCR, the reaction is performed directly on the flat surface of a glass slide that holds an array of covalently attached nested primers. The bacterial target DNA is amplified and probed using primers identifying either species-specific sequence regions of ribosomal DNA or unique bacterial target genes, such as virulence or resistance factors.

Chaotropic salts) or polysaccharides that can interact with both the nucleic acids and/or enzymes also may be potent inhibitors (32). There are also compounds that may interfere directly with the polymerase activity or compounds that modify the nucleic acids (29). By adding substances that facilitate the PCR in the presence of inhibitors, or by selectively removing inhibitors from the sample, recent developments have been achieved. The advantage of such approaches is the simplicity and speed (29).

71, 1018–1024. 43. Nogva, H. , Dromtorp, S. , and Rudi, K. (2003) Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. BioTechniques 34, 804–813. 44. , Nayak, B. , and Kogure, K. (2003) Density-dependent sorting of physiologically different cells of Vibrio parahaemolyticus. Appl. Environ. Microbiol. 69, 3569–3572. 45. Lapizco-Encinas, B. , Simmons, B. , Cummings, E. , and Fintschenko, Y. (2004) Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators.

Download PDF sample

Rated 4.77 of 5 – based on 5 votes