Enzyme Handbook by Dietmar Schomburg, Margit Salzmann (auth.)

By Dietmar Schomburg, Margit Salzmann (auth.)

Enzymes are utilized in natural synthesis and in analytical chemistry, in commercial creation strategies of prescription drugs and in foodstuff processing. discovering an appropriate enzyme for a wanted transformation or with a de- fined specificity isn't consistently a simple job. greater than 3000 enzymes are good defined up to now. The Enzyme Handbook offers the entire details for choosing the right kind enzyme to accomplish outlined alterations in a given atmosphere. The Enzyme Handbook devotes a variable variety of pages for every enzyme, looking on the volume of knowledge to be had with the EC quantity as ordering criterion inside a quantity. Revised information sheets might be published for person enzymes and newly characterised enzymes and so they can simply be looked after into the binders on the applicable position. each one information sheet is split into 7 sections: - Nomenclature (EC quantity, Systematic identify, Recom- mended identify, Synonyms, CAS Reg. No.) - Reaction and specificity (Catalysed response, response kind, average substrates, Substrate spectrum, Product spectrum, Inhibitors, Cofactors/prosthetic teams, steel compounds/salts, Turnover quantity, particular task, KM-value, pH-optimum, pH-range, Tem- perature optimal, Temperature variety) - Enzyme structure (Molecular weight, Subunits, Glyco-/Lipoprotein) - Isolation/Preparation (Source organism, resource tissue, Localisation in resource, Purification, Crystallization, Cloned, Renatured) - Stability (pH, Temperature, Oxidation, natural sol- vent, common balance details, garage) - Cross-References (to constitution facts Banks) - Literature references

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No. g. 4 Product spectrum 1 Aminoacid + dipeptide 2 L-Met + L-Met-L-Met (2] 3 L-Phe + Gly-Giy [2] 4 Leu+ Gly-Giy [6, 9, 12] 5 L-Aia + L-Aia-L-Aia 6 L-Aia + L-Aia-0-Aia 7 ? -butyl-threonylphenylalylproline [1, 2]; Co 2 + [2, 9]; Zn 2 + [2, 4, 9, 12]; Cu 2 + [2, 4, 9]; Cd 2 + [4]. g. 4 Temperature range (0 C) 3 ENZYME STRUCTURE Molecular weight 137200 (pig, tripeptidase-2, Sedimentation equilibrium centrifugation) [13] 108000 (Bacillus stearothermophilus, gel filtration) [11] 6500Q-80000 (guinea pig, gel filtration [2], Macaca fuscata, gel filtration, SOS-PAGE [6], E.

Arch. Biochem. : Eur. J. : Arch. Biochem. -Biol. : Anal. : J. : Eur. J. : Eur. J. : J. Biol. C. 37 (formerly) CASReg. No. 37290-73-6 2 REACTION AND SPECIFICITY Catalysed reaction Trans-2, 3-epoxysuccinate -+ meso-tartrate + H20-+ Reaction type Ether hydro Iysis Naturalsubstrates Trans-2, 3-epoxysuccinate + H2 0 (carbon source) [1] Substrate spectrum 1 Trans-2, 3-epoxysuccinate + H2 0 (0- and L-trans-2, 3-epoxysuccinate) [1] 2 More (acts on both optical isomers of substrate, not: cis-epoxysuccinate) [1] Product spectrum 1 Meso-tartrate 2 ?

1. 2 (formerly) Aminopeptidase M II L-Aianine aminopeptidase CASReg. No. 2 Substrate spectrum 1 Arriinoaeyl-peptide + H2 0 (free alpha-amino or alpha-imino group required [1], fast hydrolysis with Ala in N-terminal position [1], broad speeifieity for di- and tripeptides [26]) 2 Alanyl-peptides + H2 0 (hexa-or tetrapeptide hydrolysed faster than trior dipeptide [11]) 3 Ala-2-naphthylamide + H2 0 (no aeidie or basie amino aeids [11], Ala replaeed by Met, Glu, Gly, Tyr [7]) [3, 7, 11] 4 Leu-7-amido-2-methyleoumarin + H2 0 (Leu replaeed by Phe, Arg, Ala) [5] 5 S-Benzoyi-Cys-7-amido-4-methyleoumarin + H2 0 [5] 6 L-Aia-Giy (more dipeptides [9, 13], more tripeptides [3, 9, 13]) [3, 9, 13] 7 Ala-p-nitroanilide + H20 (Aia replaeed by Lys, Gly) [14] Product spectrum 1 Amino aeid + peptide 2 Ala + peptide 3 Ala + 2-naphthylamine 4 Leu + 7-amino-4-methyleoumarin 5 S-Benzoyi-Cys + 7-amino-4-methyleoumarin 6 L-Aia + Gly 7 Ala + p-nitroaniline lnhibitor(s) o-Phenanthroline; Bestatin [2, 3, 6, 9, 11]; Amastatin [3, 5, 11]; p-Hydroxymereuribenzoate [3]; EDTA [3, 7, 11, 17, 19, 28]; Co 2 + [3, 14]; Zn 2 + [3, 7]; Mn 2 + [3, 14]; Ca 2 + [14]; Leueine [3, 7]; Praline [3]; Arg-2-naphthylamide (eompetetive to Ala-4-nitroanilide) [3]; Aetinonin [5]; Ni 2 + [7]; L-Aianine [7]; L-Arginine [7]; L-Giutamine [7]; L-Methionine [7], epsilon-Amino-n-eaproie aeid [7]; Puromyein [4, 7, 15]; Hg 2 + [11]; Cu 2 + (slightly) [11]; Hydrophobieamino aeids [14]; 5, 5'-Dithiobis(2-nitrobenzoate) [14]; N-Ethylmaleimide [14]; 8-Hydroxyquinoline [15, 28]; 2, 2'-Bipyridine [15, 28]; EGTA [28]; 2, 9-Dimethyl-1, 10-phenanthroline [28]; Na2S [28]; NaN 3 [28]; KCN [28]; Ammoniumoxalate [28] Cofactor(s)/prostethic group(s) 2-Mereaptoethanol (aetivation) [5]; Dimethylsulfoxide (aetivation) [5] Meta!

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