G Protein Coupled Receptors: Structure by P. Michael Conn (Eds.)

By P. Michael Conn (Eds.)

This new quantity of Methods in Enzymology maintains the legacy of this optimum serial through containing caliber chapters authored via leaders within the box. This quantity covers G protein coupled receptors and comprises chapters on such issues as post-translation amendment of GPCR in dating to biased agonism, structure-based digital screening, and GPCR oligomerization within the brain.

  • Continues the legacy of this most excellent serial with caliber chapters authored by means of leaders within the field
  • Covers G protein coupled receptors
  • Contains chapters on such subject matters as post-translation amendment of GPCR in courting to biased agonism, structure-based digital screening, and GPCR oligomerization within the brain

Show description

Read Online or Download G Protein Coupled Receptors: Structure PDF

Similar molecular biology books

DNA: The Secret of Life

Fifty years in the past, James D. Watson, then simply twentyfour, helped release the best ongoing medical quest of our time. Now, with distinct authority and sweeping imaginative and prescient, he supplies us the 1st complete account of the genetic revolution—from Mendel’s backyard to the double helix to the sequencing of the human genome and past.

Foundations of Systematics and Biogeography

Someone drawn to comparative biology or the historical past of technological know-how will locate this myth-busting paintings certainly interesting. It attracts awareness to the seminal stories and critical advances that experience formed systematic and biogeographic pondering. It lines options in homology and type from the nineteenth century to the current in the course of the provision of a special anthology of clinical writings from Goethe, Agassiz, Owen, Naef, Zangerl and Nelson, between others.

Ion Channels

This ebook describes smooth electrophysiological innovations for recording and research of ion channels in quite a few platforms together with cells in tradition, Xenopus oocytes, mind slices, mobile organelles, and microorganisms. Many chapters take care of the prevention and correction of electrophysiological artifacts.

Multiple Sclerosis: Methods and Protocols

This certain quantity covers molecular biology, mobile biology, biomarkers, imaging, and neuropathological equipment and methods to discover a number of sclerosis (MS), with a distinct emphasis on sickness versions. With quite a bit attempt had to elucidate easy sickness mechanisms, to clone illness suitable genes, to outline novel biomarkers, and to find novel and more desirable healing and healing remedies, this booklet serves to assist researchers in attaining those huge, immense pursuits.

Extra info for G Protein Coupled Receptors: Structure

Example text

Tip: Add 1 mL of 100 Â PMSF stock directly to SDS-PAGE sample buffer aliquot before adding membranes. Detergent solubilization of membranes typically sets free protease activities that continue to function robustly in SDS-PAGE sample buffer prior to electrophoresis. Also, do not subject GPCR membrane/protein samples to heating/boiling SDS-PAGE sample buffer as membrane proteins typically aggregate upon heating. 3. , 2006). Although optimization of various parameters that affect protein production is very labor and time intensive, making the effort to do this has, thus far for 20 GPCRs, always resulted in a major improvement in production level of functional receptor.

Receptors in the left group are ordered (left to right) by decreasing an improvement factor for the combined treatment which resulted in the greatest improvement. Receptors in the right group are ordered (left to right) by decreasing an improvement factor where individual factors have the greatest effect. Species: H, human; M, mouse; P, pig; R, rat. Receptor UniProt IDs left to right: P41145, P14416, P33534, P18089, P06199, P47898, P08172, P25103, P16610, P25929, P11229, P28222, P25021, P61168, P35368, P12657, P21452, P28221, P29371, and P29274.

Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B Edited by DANIEL HERSCHLAG VOLUME 470. Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis, 2nd Edition Edited by GERALD FINK, JONATHAN WEISSMAN, AND CHRISTINE GUTHRIE VOLUME 471. Two-Component Signaling Systems, Part C Edited by MELVIN I. SIMON, BRIAN R. CRANE, AND ALEXANDRINE CRANE VOLUME 472. Single Molecule Tools, Part A: Fluorescence Based Approaches Edited by NILS G.

Download PDF sample

Rated 4.14 of 5 – based on 28 votes