By Ronald W. Dudek
High-Yield™ mobile and Molecular Biology, 3rd Edition presents the basic info wanted for USMLE Step 1 evaluate and direction research. It covers present phone and molecular biology suggestions and rules with a medical focus—what a doctor must be aware of to appreciate, diagnose, and deal with human affliction. Molecular biology is frequently taught inside of a variety of classes comparable to biochemistry, microbiology, and histology; therefore, a consolidated evaluate publication in molecular biology is mainly worthy in getting ready for the USMLE Step 1.
This version has been streamlined to take away content material coated in different High-Yield™ books equivalent to histopathology and microbiology. pictures were positioned towards the suitable textual content. New figures contain DNA melting curve and move cytometry.
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Extra resources for High-Yield Cell and Molecular Biology (3rd Edition) (High-Yield Series)
Thus, the lagging strand is lengthened. F. DNA LIGASE joins the repeats to the lagging strand and a nuclease cleaves the ends to form double-helix DNA with flush ends. G. , in skin, bone marrow, and gut), but is absent from most other somatic cells. V DNA Damage A. GENERAL FEATURES 1. Chromosomal breakage refers to breaks in chromosomes due to sunlight (or ultraviolet) irradiation, ionizing irradiation, DNA cross-linking agents, or DNA-damaging agents. qxd 9/29/10 6:49PM Page 13 aptara CHROMOSOME REPLICATION 2.
During transcription, the double helix DNA is unwound and the DNA template strand forms transient RNA–DNA hybrid with the growing RNA transcript. The other DNA strand is called the DNA nontemplate strand. 3. DNA sequences which flank the gene sequence at the 5Ј end of the template strand are called upstream sequences. DNA sequences which flank the gene sequence at the 3Ј end of the template strand are called downstream sequences. 4. Transcription is carried out by DNA-directed RNA polymerase that copies a DNA template strand in the 3Ј S 5Ј direction which in turn produces an RNA transcript in the 5Ј S 3Ј direction.
Alignment: 23 duplicated chromosomes align at the metaphase plate. 4. Disjunction: 23 duplicated chromosomes separate to form 23 single chromosomes when the centromeres split. 5. Cell division: gametes (23 single chromosomes, 1 N) are formed. II Genetic Recombination (Figure 3-2). For genetic variability to occur, DNA has the ability to undergo rearrangements by a process called genetic recombination. There are two types of genetic recombination as indicated below: A. GENERAL RECOMBINATION (Figure 3-2A) 1.