By Alfred Fahr, Xiangli Liu (auth.), Volkmar Weissig (eds.)
With approximately 100 years of in depth learn, lipids have confirmed to be an important and ever-more-promising zone of mobilephone organic learn. In Liposomes: tools and Protocols, prime specialists within the comparable fields discover state-of-the-art experimental tools regarding all points of lipids as crucial elements of the telephone membrane. Volume 2: organic Membrane Models makes a speciality of targeted tools for using liposomes in learning a number of biochemical and biophysical membrane phenomena concomitant with chapters describing an exceptional palette of cutting-edge analytical applied sciences. As a quantity within the hugely winning Methods in Molecular Biology™ sequence, the chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and warding off identified pitfalls.
Comprehensive and authoritative, Liposomes: equipment and Protocols delivers to be an important resource of sensible knowledge for each investigator, younger and pro alike, whose examine quarter consists of in a single means or one other phospholipids, glycolipids, or cholesterol.
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With approximately 100 years of in depth research, lipids have confirmed to be an important and ever-more-promising region of phone organic examine. In Liposomes: tools and Protocols, major specialists within the similar fields discover state of the art experimental equipment concerning all features of lipids as crucial parts of the mobilephone membrane.
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Extra resources for Liposomes: Methods and Protocols, Volume 2: Biological Membrane Models
1007/978-1-60761-447-0_3, © Humana Press, a part of Springer Science+Business Media, LLC 2010 21 22 Krämer, Nicklisch, and Ott In the case of hypo-osmotic stress, the major elements of stimulus response are mechanosensitive channels, which are dealt with in another chapter of this book. For the response to hyperosmotic stress, a major reaction is the activation of transport systems leading to the accumulation of compatible solutes. On the other hand, membrane bound sensory systems for hyperosmotic stress must be available, leading to appropriate responses in the cell on the level of transcription.
I. Investigations of protein-protein interactions of cytochrome P-450 in phospholipid vesicles and liver microsomes. J Biol Chem 257:7023–7029 24. Schwarz D, Pirrwitz J, Ruckpaul K (1982) Rotational diffusion of cytochrome P-450 in the microsomal membrane-evidence for a clusterlike organization from saturation transfer electron paramagnetic resonance spectroscopy. Arch Biochem Biophys 216: 322–328 25. Taniguchi H, Imai Y, Sato R (1987) Proteinprotein and lipid-protein interactions in a reconstituted cytochrome P-450 dependent 26.
The role of bilayer thickness and the concept of hydrophobic mismatch in MscL functionality have further been investigated in studies exploring the effect of static magnetic fields (SMF) of moderate intensity (~400 mT) on the open probability of MscL reconstituted in phospholipid bilayers (15, 16). Results of these studies suggest that due to co-operative superdiamagnetism of phospholipid molecules, SMF most likely cause a rearrangement of the lipids resulting in a change of bilayer thickness.