By Jason X.-J. Yuan
This quantity provides a world assessment of the function of ion flux through transmembrane ion channel proteins within the legislation of pulmonary vascular tone and within the vascular home improvement procedures linked to pulmonary vascular disease-offering a entire evaluate of the a number of households of ion channels which have been pointed out and characterised in pulmonary artery soft muscle, in addition to a realistic dialogue of experimental instruments for the learn of ion channel body structure and molecular biology.
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Extra resources for Lung Biology in Health & Disease Volume 197 Ion Channels in the Pulmonary Vasculature
311 II. Properties of Unitary Ca2þ-Activated ClÀ Channels in Pulmonary Artery Smooth Muscle Cells . . 312 III. Regulation of Ca2+-Activated ClÀ Channels . . 316 IV. Pharmacology of Ca2+-Activated ClÀ Channels . . 317 V. Function of Ca2+-Activated ClÀ Channels in the Pulmonary Vasculature . . 322 VI. Molecular Biology of Ca2+-Activated ClÀChannels . . 325 VII. Conclusions . . 329 References . . 330 16. Volume-Sensitive ClÀ Channels in Pulmonary Vascular Smooth Muscle and Endothelial Cells .
Microvascular Versus Macrovascular Endothelial Permeability . . 474 VIII. Summary and Future Directions . . 477 References . . 478 23. Role of Ca2+ Sparks in the Regulation of Pulmonary Vascular Tone . . . 483 James S. K. Sham, Anita Umesh, Xiao-Ru Yang, and Mo-Jun Lin I. Introduction . . 483 II. Ryanodine Receptor: the Mediator of Ca2þ Sparks . . 484 III. Ca2+ Sparks of Systemic Vascular Smooth Muscle . . 492 xxii Contents IV. Ca2+ Sparks of PASMCs . . 494 V. Physiological Roles of Ca2+ Spark in PASMCs .
Expression of Foreign Genes Using Mammalian Expression Systems . . 688 IV. DNA Sequence Alterations . . 691 V. Analysis of Expression and Function of Heterologous Ion Channels . . 701 VI. Application of Site-Directed Mutagenesis for Study of Ion Channel Functions . . 704 VII. Summary . . 706 References . . 707 Contents xxv 35. Screening for mRNA Expression Using the Polymerase Chain Reaction . . 711 Ivana Fantozzi, Carmelle V. -J. Yuan I. Introduction . . 711 II. The Polymerase Chain Reaction Technique .