Molecular Methods in Developmental Biology: Xenopus & by Guille, Matt

By Guille, Matt

Show description

Read Online or Download Molecular Methods in Developmental Biology: Xenopus & Zebrafish (Methods in Molecular Biology Vol 127) PDF

Best biology books

Translation Initiation: Extract Systems and Molecular Genetics

For over fifty years the equipment in Enzymology sequence has been the significantly aclaimed laboratory ordinary and essentially the most revered courses within the box of biochemistry. The hugely appropriate fabric makes it a vital ebook for researchers in all fields of lifestyles and comparable sciences.

Evolutionary biology of ostracoda: its fundamentals and applications: Proceedings of the Ninth International Symposium on Ostracoda

There are lots of aggressive works out there bearing on evolutionary biology, yet this quantity is kind of designated in its idiographic point targeting Ostracoda seen from a variety of disciplines, a long time and environments. The publication bargains with quite a few strains of idiographic biology and palaeontology of Ostracoda and nomothetic trials focusing strongly on evolutionary biology.

Liposomes: Methods and Protocols, Volume 2: Biological Membrane Models

With approximately 100 years of in depth examine, lipids have confirmed to be a necessary and ever-more-promising sector of cellphone organic study. In Liposomes: equipment and Protocols, top specialists within the comparable fields discover state-of-the-art experimental equipment related to all elements of lipids as crucial parts of the cellphone membrane.

Fungi : biology and applications

Fungi: Biology and functions, moment Edition€provides a complete remedy of fungi, protecting biochemistry, genetics and the clinical and monetary importance of those organisms at introductory point. € with out earlier wisdom of the topic assumed, the hole chapters supply a huge evaluate of the fundamentals of fungal biology, particularly the body structure and genetics of fungi and likewise a brand new bankruptcy at the software of genomics to fungi.

Additional info for Molecular Methods in Developmental Biology: Xenopus & Zebrafish (Methods in Molecular Biology Vol 127)

Sample text

Biol. 37, 291–298. 9. , and Takahashi, Y. (1997) Mesodermal subdivision along the mediolateral axis in the chicken controlled by different concentrations of BMP-4. Development 124, 1975–1984. 10. , and Takeda, H. (1997) Specification of posterior midbrain region in zebrafish neuroepithelium. Genes Cells 1, 369–377. 11. Tung, T. , Chang, C. , and Tung, Y. F. Y. (1945) Experiments on the developmental potencies of blastoderms and fragments of Teleostean eggs separated latitudianally. Proc. Zool. Sci.

Introduction When characterizing the developmental expression of a novel gene, or when examining the response of a known gene to experimental manipulations, it is important to be able to assay mRNA transcript levels accurately. Although a number of techniques for transcript analysis are available, one of the most useful and widespread is the ribonuclease (RNase) protection assay (for example, see Fig. 1). The major advantages of RNase protection analysis are good sensitivity, excellent specificity, and the linear response to differing transcript levels.

9. Incubate at 37°C for 30 min. 10. RNase inactivation is accomplished by the addition of 10 µL of 10% SDS and 2 µL of Proteinase K solution, followed by incubation for 15 min at 37°C. 11. Extract all samples with an equal volume of phenol–chloroform. 12. 5 Ribonuclease Protection Analysis 35 volumes of ethanol and 10 µg of carrier RNA. Salt is already present in the digestion buffer. 13. Recover the RNA by centrifugation for 10 min in a microfuge and then resuspend the pellet in 5 µL of formamide loading buffer.

Download PDF sample

Rated 4.33 of 5 – based on 19 votes