By Gavin Burnell, Geoff Allan
With wild shares declining as a result of over-fishing, aquaculture may have a extra major function to play in assembly destiny call for for clean fish. advancements in examine proceed to steer to advancements in aquaculture construction platforms, leading to elevated creation potency, better product caliber for shoppers and a extra sustainable undefined. New applied sciences in aquaculture studies crucial advances in those components. half one specializes in the genetic development of farmed species and keep an eye on of copy, with chapters on genome-based applied sciences in aquaculture learn, selective breeding and the creation of unmarried intercourse and sterile populations, between different subject matters. components and 3 assessment key concerns in overall healthiness, nutrition and husbandry, akin to the keep an eye on of viral and parasitic ailments, nutrition and husbandry thoughts to enhance affliction resistance, advances in diets for specific fish species and the influence of damaging algal bloom on shellfisheries aquaculture. Chapters in elements 3 and 4 then learn the layout of alternative aquaculture creation structures, together with offshore applied sciences, tank-based recirculating platforms and ponds, and key environmental concerns, equivalent to the prediction and review of the influence of aquaculture. Concluding chapters specialise in farming new species. With its famous editors and distinct foreign group of participants, New applied sciences in aquaculture is a vital buy for execs and researchers within the aquaculture undefined.
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Additional info for New Technologies in Aquaculture: Improving Production Efficiency, Quality and Environmental Management (Woodhead Publishing Series in Food Science, Technology and Nutrition)
The major weakness of AFLP markers is their dominant nature of inheritance. Genetic information is limited with dominant markers because essentially only one allele is scored; and at the same time, since the true alternative allele is scored as a different locus, AFLP also inflates the number of loci under study. As dominant markers, information transfer across laboratories is difficult. In addition, AFLP is more technically demanding, requiring special equipment such as automated DNA sequencers for optimal operations.
As with all next generation sequencing platforms, the SOLiD sequencing does not require cloning of genomic DNA. It starts with the creation of a ‘library’ by ligation of two adaptors to sheared genomic DNA (Fig. 3). Once the adapters are ligated to the library, emulsion PCR is conducted using the common primers to generate ‘bead clones’ in which each contains a single nucleic acid species. Each bead is then attached to the surface of a flow cell (microscope slide) via 3′ modifications to the DNA strands (Fig.
For genetic resource analysis, the number of primer combinations required for construction of phylogenetic trees/dendrograms depends on the level of polymorphism in the populations, but probably takes no more than five to ten primer combinations. AFLP combines the strengths of RFLP and RAPD. It is a PCR-based approach requiring only a small amount of starting DNA; it does not require any prior genetic information or probes; and it overcomes the problem of low reproducibility inherent to RAPD. AFLP is capable of producing far greater numbers of polymorphic bands than RAPD in a single analysis, significantly reducing costs and making possible the genetic analysis of closely-related populations.